Genetic engineering of VHH-with-multiple-paratopes (Single domain with multidomain antibody megabody) Targeting Multiple (TM) cytokines in the osteoarthtis joints as a potential of DMOAD

Title : Genetic engineering of VHH-with-multiple-paratopes (Single domain with multidomain antibody megabody) Targeting Multiple (TM) cytokines in the osteoarthtis joints as a potential of DMOAD

Abstract:

VHH is a single-domain antibody that can bind to the epitope(s) of its target antigen(s) in a very efficient way due to its tiny size. Osteoarthritis is a chronic, debilitating disease affecting hundreds of millions of people around the globe. There are no disease- modifying drugs or treatments available, except for knee arthroplasty. We conducted in vitro, in vivo, and animal studies on using multidomain single-domain antibodies (or we called it a megabody(™)) that targeted TNF-Alpha, IL-6, IL-1beta, NGF, MMP-13, ADAMST-4 and IKK- Alpha-Beta-forming a hepta-tropes as a potential DMOAD. To enhance the duration of the megabody(™) in the intraarticular, we engage a nanoparticle consist of PLGA (Poly-lacto- Gycolic-acid) with 75:25 percentage w/w, respectively.

Biography:

Dr. Johan graduated from Universitas Sriwijaya, medical school in 1998, and after his internship (housemanship) in 2000, he worked in a rural area for one year and was posted in the Army General Hospital as a medical officer thereafter, before getting his specialist training in the National University of Singapore. He completed his Master of Medicine and Master of Surgery in 2009, and continued his general surgical training in Singapore and passed his MRCP(UK) exam and MRCS exam. He obtained his fellowship in orthopedics and trauma (FRCS) in Glasgow in 2014. Besides his busy clinic schedule, he immersed himself in the research work that has been published in multiple journals. He has been granted multiple patents by Intellectual Property of Singapore (IPOS) for his enormous works in cellular and biologic therapy and he is still pursuing his passion in both clinical practice and research to this day.