Title : Rhizoma drynariae extracellular vesicles promote osteogenic differentiation of human bone marrow mesenchymal stem cells via the estrogen receptor pathway
Abstract:
Extracellular vesicles(EVs) originating from plant have been exploited for treating many diseases. Rhizoma Drynariae(RD) is a natural plant herb with clear clinical efficacy against osteoporosis, but has some side effects. Here, we analyzed whether Rhizoma Drynariae-derived extracellular vesicles(RDEVS) could be targeted to enrich in bone tissue or cell and enhance anti-osteoporosis therapeutic effect. RDEVs were isolated from fresh Rhizoma Drynariae by juice and differentially centrifuge-based methods. RDEVs are enrich with nucleic acids, proteins and lipids. RDEVs can be internalized by human bone marrow mesenchymal stem cells (hBMSCs) and promote osteogenic differentiation. In the presence of estrogen inhibitor, the osteogenic differentiation of hBMSCs is diminished, which RDEVs can reverse.The estrogen receptor signaling pathway could be a possible mechanism of action. Using ovariectomized mice model, we demonstrated that RDEVs can also target bone tissue in vivo and have the effect of enhancing bone mineral density in osteoporotic mice. The data in vivo and vitro suggest that RDEVs have the potential to target bone tissue enrichment and treat osteoporosis.
